Characteristics of saline-alkali-tolerant and petroleum hydrocarbon-degrading bacteria isolated from petroleum oil-polluted soil in Momoge wetland

In situ screening of saline-alkali-tolerant and petroleum hydrocarbon-degrading bacteria can be used to remediate the saline-alkaline soils contaminated with petroleum.In this study, a polycyclic aromatic hydrocarbon degrading strain was isolated from the oil-contaminated soil of the Momoge wetland with phenanthrene (Phe) as the sole carbon source.The morphology and 16S rRNA gene sequence analysis were identified.The degradation characteristics and degradation kinetics of the phenanthrene strain were systematically studied, which intended to provide a good solution for the treatment of wetland petroleum pollution.The morphological and phylogenetic tree analysis indicated that the isolated strain belonged to Halomonas, assigned as Halomonas sp.S1-8.The optimum salt concentration of strain S1-8 for Phe degradation was (NaCl)=5%, pH=8.0, temperature=30 ℃.The degradation kinetics of strain S1-8 to Phe under saline-alkali stress was in accordance with the first-order degradation kinetics model.Strain S1-8 had a wide range of adaptation to salt concentration and pH, respectively.Therefore, Halomonas sp.S1-8 has a potential application prospect in bioremediation of oil field pollution.