Isolation and Identification of Low-temperature Degrading Strains for Corn Stubble

Using samples collected from hilly land of Wei Yuan county of Gansu province, a cellulose-degrading fungi strain D5 was isolated through methods of Congon red stain and liquid shake-flask fermentation at 15℃. The cellulase activity and corn stubble degrading ability of strain D5 were determined, and strain D5 was classified according to the analysis of its 18S rRNA gene sequences. The result showed that Xylanase, CMCase and filter paper activity(FPA) of strain D5 were 52.7 U·mL^-1, 31.5 U·mL^-1 and 29.6 U·mL^-1, respectively;Strain D5 possessed high corn stubble degrading ability with degrading rate of 29.8% in 10 days. The strain D5 was identified as Penicillium sp. by ITS sequence analysis, with tremendous potential for degrading corn stubble.