Abstract: In order to screen out soil amendments suitable for improving red clay soil with the flue-cured tobacco planting in Ruyuan tobacco planting area of South China, six treatments were set up: control (CK), B1 (rice straw biochar), B2 (bagasse), B3 (rapeseed cake fertilizer), B4 (rice straw), B5 (bio-organic fertilizer). The effects of different organic materials on physicochemical properties and bacterial community of Ruyuan tobacco planting soil were studied. The results show that B1, B3 and B4 treatments significantly increase soil pH but reduce soil bulk density compared with CK. B1 treatment increases the contents of total phosphorus, available phosphorus and available potassium by 31.1%, 49.8% and 112% compared with CK, respectively. B1 and B2 treatments increase the proportion of large aggregates, average weight diameter and water-stable aggregates by 73.7% and 47.4%, 35.7% and 23.1%, 15.0% and 10.6%, respectively. Compared with CK, B1 treatment and B2 treatment significantly increases the organic carbon and microbial biomass carbon by 68.9% and 21.7%. The catalase, alkaline phosphatase and sucrase activities in B1 and B2 treatments are significantly higher than those in CK. The input of organic materials increases the bacterial abundance of Proteobacteria and Gemmatimonadetes in soil, and B2 treatment exhibits the highest species richness index. The addition of organic materials could significantly increase soil nutrients, improve the stability of soil aggregates, and significantly increase soil enzyme activity, bacterial abundance and alpha diversity. Among them, rice straw biochar and bagasse demonstrate better effects. It is recommended that rice straw biochar and bagasse should be preferentially selected as soil amendments during flue-cured tobacco planting in Ruyuan tobacco-growing area of South China.