Abstract: To investigate the control effect of three plant immune inducers against soybean root and seed diseases as well as their effects on soybean growth and yield, soybean (cultivar Dongsheng 1) seed germination tests in laboratory, pot experiments in greenhouse and plot trials in field were carried out. The results showed that seed germination and growth are not negatively impacted coated with the three tested concentrations of the three plant immune inducers, and optimal concentrations could promote germination. Control effect of oligosaccharides on soybean cyst nematodes is 50.4% in greenhouse under quantitative inoculation, and its control effect on soybean root rot is 72.6% in field. Additionally, nitrogen fixation ability of soybean roots could be improved by Puliwei and Wokesen, from metabolite of Mortierella alpina and Metasequoia glyptostroboides, respectively. The three plant immune inducers could control the occurrence of seed diseases by over 42.2% with yield increased over 5.35%. Soybean ripening is accelerated by every preparation, with oligosaccharides resulting in thicker stalks and better lodging resistance. The better yield increase and disease prevention of oligosaccharides exhibits its potential for application.